Equipment

OMX-SR 3D-SIM Super-Resolution System

Offering resolution at twice the diffraction limit of traditional microscopes, the DeltaVision|OMX system is the practical solution for three dimensional super resolution imaging that does not depend on special fluorophores or fluorescent proteins. The OMX is also set-up for TIRF and fast conventional imaging of live cells.

Features of the OMX-SR 3D-SIM Super-Resolution System

  • Structured Illumination mode for high-resolution imaging. X,Y resolution to ~ 100nm, Z resolution to ~ 300nm.
  • Conventional Illumination mode for fast live cell imaging. Up to 65 frames/second in three colors.
  • Lasers lines (405, 488, 561, 642) and and emission filters (419-465, 500-550, 609-654, 665-705) for imaging DAPI, green, red and far red fluorophores.
  • Ring TIRF.
  • Three PCO Edge 5.5 sCMOS cameras allow simultaneous three color imaging.
  • Environmental control for live imaging.
  • Controlled by AquireSR software, image processing by SoftWorx imaging software.

More information and resources for the OMX-SR 3D-SIM Super-Resolution System

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BD Pathway 855 High-Content Bioimager

Purchased September 2008, the 855 is a versatile system for high-content confocal imaging of live or fixed samples. It can handle specimen formats from single slides to multiwell plates. It can automatically image multiple sites per slide or well and every well of a multiwall plate. The 855 includes powerful imaging software to perform a broad range of fluorescence-based kinetic and endpoint biological assays.

Features of the BD Pathway 855 High-Content Bioimager

  • Confocal fluorescence, widefield fluorescence, brightfield.
  • X-cite lamps to provide full spectrum illumination (340nm-700nm).
  • Hamamatsu Orca ER cooled CCD camera. - Handles slides to multiwell plates.
  • Laser and image based autofocus.
  • Environmental control. - Liquid handling.
  • Robotics to handle loading/changing of multiwell plates.
  • BD Pathway imaging software and BD AttoVision Software for image processing and analysis.

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BD Pathway 435 High-Content Bioimager

Also acquired September 2008, the 435 is similar to the 855, but specialized for imaging fixed specimens. It can handle specimen formats from single slides to multiwell plates. It can automatically image multiple sites per slide or well and every well of a multiwall plate. Both machines include the same powerful imaging software to perform a broad range of fluorescence-based kinetic and endpoint biological assays.

Features of the BD Pathway 435 High-Content Bioimager

  • Confocal fluorescence, widefield fluorescence, brightfield.
  • X-cite lamp to provide full spectrum illumination (340nm-700nm).
  • Hamamatsu Orca ER cooled CCD camera.
  • Handles slides to multiwell plates.
  • Laser and image based autofocus.
  • BD Pathway imaging software and BD AttoVision Software for image processing and analysis.

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Leica SP8 Scanning Confocal

Installed in January of 2016, the SP8 is a versatile scanning confocal with all the bells and whistles. This system has 4 sensitive HyD detectors and 2 PMT detectors enabling the simultaneous imaging of up to 6 different wavelengths. It has a White Light Laser (WLL) that can provide up to 8 excitation lines between 470 - 670nm) in addition to 405nm and 440nm lasers to provide almost any excitation wavelength and a prism spectrometer to control emission wavelengths to make this a very versatile system compatible with most combinations of fluorophores.

Features of the Leica SP8 Scanning Confocal

  • Leica DMi8 inverted microscope platform
  • Lasers: WLL (470-670nm), 405nm, 440nm.
  • Can simultaneously image fluorescence and brightfield or DIC
  • FRAP or FRET fully supported with rapid kinetic analysis
  • Picoquant system for FLIM or FCS
  • Huygens deconvolution software
  • Controlled by Leica LAS-X software

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Leica SP5 Scanning Confocal

For more SP5 information and resources. Installed June 2008, this system has 4 sensitive detectors enabling the simultaneous imaging of up to 4 different wavelengths. It has 3 lasers providing 7 excitation wavelengths and a prism spectrometer to control emission wavelengths to make this a very versatile system compatible with most combinations of fluorophores.

Features of the Leica SP5 Scanning Confocal

  • Leica DMI 6000 CS inverted microscope platform
  • 7 lines of laser illumination (458,476,488,496,514,561 and 633nm) can excite from CFP to CY-5 fluorophores
  • Can simultaneously image fluorescence and brightfield or DIC
  • FRAP or FRET fully supported with rapid kinetic analysis
  • Controlled by Leica LAS software

More information and resources for the Leica SP5 Scanning Confocal

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Olympus OSR Spinning Disk Confocal

The OSR microscope achieves super resolution by combining superior optics with the new Yokogawa W1 Spinning Disk to improve signal and reduce noise. In super resolution mode, Airy disk oversampling drives resolution down to 120 nm by capturing subtle high-frequency special components not visible in conventional spinning disk microscopy.

Features of the Olympus OSR Spinning Disk Confocal

  • Hammamatsu Flash 4 V2 camera (100fps full frame, 2048x2048, 82%QE)
  • 4 laser diodes (445nm, 488nm, 514nm, 561nm)
  • Zero-drift autofocus capability
  • Can also collect bright-field and DIC images
  • Tokai Hit Stage Top Incubation system

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Nikon A1 Scanning Confocal/ TIRF Microscope

Installed May 2012, this versitile system is built on the Nikon Eclipse Ti inverted microscope. It includes the A1 scanning confocal with four lasers that provide five excitation wavelengths, four sensitive fluorescence detectors and can image in both standard or spectral mode. This microscope includes the Perfect Focus system to maintain focus throughout long-term live imaging experiments. Also included are a very nice camera and a TIRF module for imaging high resolution fluorescence in regions very near the coverslip.

Features of the Nikon A1 Scanning Confocal/TIRF Microscope

  • Nikon Eclipse Ti inverted microscope platform
  • The Nikon Perfect Focus system
  • Five laser lines(447, 488, 514, 561, and 640nm) to excite many of the popular fluorophores from CFP to Cy5
  • Simultaneous or sequential multichannel imaging
  • Standard filter based imaging or Spectral detector for added flexabilty in the separation of fluorophores
  • TIRF module with a 100X TIRF objective
  • Hammamatsu Orca-Flash 4.0 sCMOS camera
  • Controlled by Nikon Elements software

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DeltaVision Personal DV

The Applied Precision DeltaVision personalDV Live Cell Imaging System is optimized for deconvolution microscopy. Deconvolution is a software technique that mathematically de-blurs images. This system features a very nice motorized stage for precise control of X, Y, and Z plane movements. Multiple sites on a slide can be marked and revisited.

Features of the DeltaVision Personal DV

  • Olympus IX-71 Customized Inverted Microscope Stand
  • Photometrics CoolSNAP HQ2 CCD camera
  • Insight Solid State Illumination system with filters for DAPI, CFP, GFP/FITC, YFP, RD-TR-PE, mCherry/Alexa594, Cy5 fluorophores
  • Can also collect bright-field and DIC images
  • WeatherStation Environmental Chamber to control temperature and carbon dioxide levels for live cell imaging
  • Controlled by SoftWorx imaging software

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Nikon NiE

Installed May 2012. The Nikon NiE is a very nice general purpose research microscope. It is equipped for brightfield, DIC, phase and epifluorescence imaging. The microscope has 4X to 100X objectives so a wide range of samples can be accommodated. Epifluorescence imaging on this scope works best with thin, flat specimens.

Features of the Nikon NiE

  • The Nikon NiE is an upright, semi-automated microscope platform
  • Hamamatsu Orca-Flash 2.8 sCMOS high resolution camera
  • Lumencor SpectraX light source for fluorescence imaging of UV/DAPI, CFP, Green, YFP, Red, and Far-red (CY-5) fluorophores
  • Motorized focus control for Z-stack images
  • Controlled by Nikon Elements software

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Motic EasyScan

A slide scanning microscope for brightfield imaging. This scope will automatically image whole slides or parts of slides. It is great for histochemically stained samples. Can load up to six slides.

Features of the Motic EasyScan

  • 2X - 40X magnification
  • Autofocus
  • Modes: Normal, High precision, Extended Depth of Field, Z-Stack.
  • Slide dimension must be 76mm x 26mm (standard) with a #1.5 coverslip

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Zeiss Axioplan

The Axioplan is a good general purpose research microscope. It is equipped for brightfield, DIC, and epifluorescence imaging. Images are captured using an IMI Tech IMC-3145FT digital color camera controlled by basic imaging software. This software, while basic, is easy to learn and easy to use. This microscope is on a cart and is available for use in the imaging center or may be used in your lab or classroom.

Features of the Zeiss Axioplan

  • The Zeiss Axioplan is an upright microscope platform
  • IMI Tech IMC-3145FT color camera
  • Hg Lamp illumination with filters for UV/DAPI, Green and Red fluorophores
  • Easy to use, a great option for basic imaging or just to look around
  • On a cart - may be used in your lab or classroom

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Veritas Microdissection System

The Veritas is a fully automated microscope system designed to dissect specific cells, organelles, or tissues from your specimen for further analysis. It can do both laser-cutting for a large number of cells and laser capture for single cells or organelles.

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Leica M205FA Stereo Microscope

This is an excellent microscope for a wide range of uses - from specimen preparation and screening to high-resolution imaging of a wide range of samples. It is equiped for brightfield and epifluorescense imaging. Images are captured using the Leica DFC310FX color camera and Leica LAS software.

Features of the Leica M205FA Stereo Microscope

  • Hg lamp with filters for UV/DAPI, Green and Red fluorophores
  • Magnification from 7.8X to 160X (eyepiece)
  • Automated focus and filter controls

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